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Capabilities

Cell Isolation and Systems Analysis

The Cell Isolation & Systems Analysis (CISA) capability at EMSL includes techniques for isolating cells from complex cell populations or environmental samples for further 'omics and imaging analyses. EMSL specializes in quantitative live cell fluorescence imaging with single molecule sensitivity, super resolution fluorescence and electron microscopy techniques, and transcriptomics and proteomics analyses. These capabilities provide the foundation for attaining a molecular-level understanding of protein functions and microbial-community dynamics to enable molecular level investigations of single cells and the pursuit of systems biology.

Capability Detail

Photo of researcher in laboratory
  1. Continuous Precipitation of Ceria Nanoparticles from a Continuous Flow Micromixer.
  2. Chemical Characterization of Individual Particles and Residuals of Cloud Droplets and Ice Crystals Collected On Board Research Aircraft in the ISDAC 2008 Study.
  3. Effect of combined local variations in elastic and inelastic energylosses on the morphology of tracks in ion-irradiated materials.
  4. Evaluation of selected binding domains for the analysis of ubiquitinated proteomes.
  5. Oxidative Dissolution of UO2 in a Simulated Groundwater Containing Synthetic Nanocrystalline Mackinawite.
  1. Predictive model a step toward using bacteria as a renewable fuel source (Green isoprene)
  2. Examining the core components of Arctic clouds to clear up their influence on climate (Forecast calls for better models)
  3. Sulfide and iron work together to reveal a new path for radionuclide sequestration (Sulfur cleans up)
  4. First view of micellar bundles revealed by EMSL electron microscopy tools, techniques (Micelle microscopy)
  5. Biofilms move electrons long distances across two distinct layers, even under starving conditions (Long distance)

Cell Isolation and Systems Analysis Capabilities Available at EMSL

To help with proposal planning, icons in the table below indicate instrument availability:

  • 10 hours a day, 5 days a week
  • 24 hours a day, 7 days a week
Instrument Contact
Linggi, Bryan E
Markillie,Lye Meng
Orr, Galya
Arey, Bruce
Dohnalkova, Alice
  • Electron Microscope: Transmission, Liquid Helium, Cryo (JEOL) - (avail. Mar. 2013)
Evans, James
Chrisler,William B
Orr, Galya
Hu, Dehong
Orr, Galya
  • Microbial Bioreactors
Linggi, Bryan E
Hu, Dehong
Orr, Galya
  • Microscope: Fluorescence, Confocal, Real-Time
Orr, Galya
Hu, Dehong
Orr, Galya
Hu, Dehong
Orr, Galya
Orr, Galya
Jiang, Weilin
Lea, Scott
Shutthanandan, Shuttha
Hu, Dehong
Cell Isolation and Systems Analysis Capability Lead: Galya Orr | , 509-371-6127