NMR Spectrometer: 500 MHz WB Bruker Advance Imaging
Quick Specs
- 500-MHz frequency
- 11.7-tesla magnet
- 72-mm vertical bore
- Coupled to microscopy capabilities
The Bruker Avance spectrometer is the latest addition to thesuite of nuclear magnetic resonance (NMR) spectrometers located at EMSL. This MR microscopy (MRM) spectrometer is instrumental in helping EMSL researchers address and/or achieve the following objectives:
- Developing MRM imaging and localized spectroscopy that will achieve the highest possible spatial and spectral resolution in the shortest possible time. To achieve these goals, four approaches are being explored by EMSL researchers: (1) MRM where spectral resolution is increased via a combination of slow magic angle spinning (MAS) and special radiofrequency pulse sequences; (2) integrated MRM and optical (confocal) microscopy, which makes it possible to study cell systems simultaneously with two different image modalities; (3) MRM using small NMR coils; and (4) MRM in large external fields.
- Applying MRM capabilities to in vitro biological and biomedical research on prokaryotic and eukaryotic cell systems and to in vivo biomedical research on small animals (mice).
System Configuration and Operational Overview
The Bruker Avance 500-MHz Wide Bore NMR Spectrometer consists of an:
- Actively shielded, vibration-damped 72-mm vertical bore, 11.7-Tesla magnet
- Avance model digital spectrometer that provides state-of-the-art rapid imaging, improved experiment planning, and digital signal generation and processing capabilities.
Imaging hardware includes a Bruker Mini0.5 actively-shielded gradient probe with an animal (mouse) handling system and 38-mm inside-diameter radiofrequency resonator. The gradient strengths are 20 Gauss-per-centimeter in each of three orthogonal directions. Currently, a slow-MAS probe with an outside diameter of 38 mm is under construction that can be inserted into the gradient probe.
Microscopy hardware includes a homebuilt combined confocal and magnetic resonance microscope. This consists of a 250 Gauss-per-centimeter gradient probe for ~10-micron spatial resolution; an in-magnet confocal scanning fluorescence microscope with multispectral (two channel) detection and ~1-micron spatial resolution; and a perfusable, temperature-controlled, integrated radiofrequency probe/sample chambers for the observation of live cells in two configurations (150 to 800 micron-diameter single cells or cell agglomerates, or cell monolayers adhering to a 5-mm glass cover slip).
Finally, the spectrometer is equipped with a liquid-state 1H, 19F, 31P, and 13C 10-mm QNP probe. The inner coil can be switched via acquisition software between carbon, phosphorus, or fluorine, and an outer coil is used for proton observe or simultaneous proton decoupling. This probe does not have gradient capabilities.
All Related Publications Related Publications
- Optimization of nanoparticle core size for magnetic particle imaging.
- Metabolomics in Lung Inflammation: A High Resolution ¹H NMR Study of Mice Exposed to Silica Dust .
- NMR bioreactor development for live in-situ microbial functional analysis.
- Correlated Biofilm Imaging, Transport and Metabolism Measurements via Combined Nuclear Magnetic Resonance and Confocal Microscopy .
- Investigations of Structure and Metabolism within Shewanella oneidensis MR-1 Biofilms .
