Tissue-Culture Facility
At EMSL, researchers use the single-molecule fluorescence/patch clamp microscope to combine high-sensitivity fluorescence imaging simultaneously with physiological measurements to identify real-time molecular interactions and conformational changes of cell-membrane receptors and their functional consequences.
The study of individual proteins in the membrane of living cells is enabled by the presence of a tissue culture facility adjacent to the microscopes. The facility supports the growth of eukaryotic primary cells or cell-lines, including human cell lines. In addition to a bio-hood, the facility includes CO2 incubators, a centrifuge, 37°C water bath and a shaker.
All Related Publications Related Publications
- Syndecan-1 Mediates the Coupling of Positively Charged Submicrometer Amorphous Silica Particles with Actin Filaments across the Alveolar Epithelial Cell Membrane.
- Identification and Localization of the CupB Protein Involved in Constitutive CO₂ uptake in the Cyanobacterium, Synechocystis sp. Strain PCC 6803.
- Submicron and Nanoscale Inorganic Particles Exploit the Actin Machinery to be Propelled Along Microvillilike Structures into Alveolar Cells.
- Parsing ERK Activation Reveals Quantitatively Equivalent Contributions From Epidermal Growth Factor Receptor and HER2 In Human Mammary Epithelial Cells.
- Cholesterol Dictates the Freedom of EGF Receptors and HER2 in the Plane of the Membrane.
