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The instrument is a high-performance Fourier-transform ion cycloton resonance (FTICR) mass spectrometer, which was designed for high-throughput automation, accurate mass MSn, elemental composition assignments and LC/FTMS with <2ppm mass accuracy. The mass spectrometer is equipped with an electrospray ionization (ESI) ion source and is coupled to an actively shielded 9.4 tesla magnet. This mass spectrometer has a mass range of 29 to 66,000 Daltons with electrospray ionization and an (ESI internal calibration) mass accuracy of <1 ppm for protein samples ranging from m/z 100 to 800 Daltons. The (ESI external calibration) mass accuracy is <1.5ppm for parent ion peaks from angiotensin I at m/z 432 and m/z 648. The mass resolution of the instrument using an ESI source is 900,000 for ubiquitin at m/z 857. The sensitivity of the instrument for ESI on Ubiquitin (m/z 780) is S/N > 10:1 for 200 fmol of consumed protein. The vacuum system consists of three stages of differential pumping, which gradually decrease the pressure from atmospheric to ultra-high vacuum (1 E-9 mbar) in the region of the FTICR trap. The spectrometer utilizes a commercial Windows NT data station and software containing BioTOOLSTM (for the analysis of MS and MS/MS of biomolecules) and MASCOT package for DNA and protein data base searching for applications in proteomics. For more information on this instrument contact Ljiljana Pasa Tolic or Harold R. Udseth. |
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Kim K. Hixson Updated: August 7, 2001 Security and Privacy |
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