Developing the proteome network regulating structure and function of root-development of switchgrass on marginal lands
EMSL Project ID
60294
Abstract
Abstract: Switchgrass (Panicum virgatum) is a leading biomass perrenial grass native to the United States. Switchgrass plants are known for tolerance of poor soil conditions, mainly due to its deep and strong root system. Our long-term studies showed that the root-tips have a very strong regenerative capacity, new root-tips emerged from shrunken roots as soon as soil moisture recovered after long term drought. The plants are also tolerant of acidic soil and high concentration of toxic aluminum ions (as high as 100 µM Al 3+ ion activity) which mainly cause injuries in root-tip regions. The activity of the root-tips has a vital role in developing a functional root system. Root-tip represents a biological system with a continuity of cell division, cell differentiation and cell growth. Switchgrass root-tips contain a closed root apical meristem (RAM), where the origins of each layer of cells can be traced back to the initials. In this project, we will select drought and Al-treated switchgrass plants, to profile proteomes in each distinct types of cells found in root-tips. These cells include (but is not necessarily limited to) the cells of the quiescent center (stem cells of RAM), the initials for every file of cells, the cells undergoing division and at different stages of differentiation, and cells with defined functions, such as the epidermis, root caps, elongation cells, etc. Models associating proteome expression with each distinct cell types and those regulating/coordinating key cellular activities in root-tips will be developed. Results expected from this project will greatly advance our understanding of the biological processes that regulate/coordinate cell division, cell differentiation and cell growth in the root-tip which, ultimately, determines the size, function and spatial distribution of the root system in soil. This project will be conducted through collaboration among Tennessee State University, USDA-ARS, and DOE-EMSL
Project Details
Project type
Large-Scale EMSL Research
Start Date
2022-10-01
End Date
N/A
Status
Active
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
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