PhenoAccess: Physiological Phenotyping of Brachypodium Accessions
EMSL Project ID
50010
Abstract
Although the effects of elevated CO2 on plant growth, physiology and metabolism has been investigated thoroughly, the underlying integrated organismal, cellular and molecular mechanisms of these changes are less understood. Also, two major plant photosynthesis types i.e. C3 and C4 plants, are often affected by all global change parameters in a manner that is distinct from each other. In this study, accessions of Brachypodium distachyon Bd21 (C3 model grass) and Setaria viridis A10.1 (C4 model grass) will be grown under current and elevated CO2 levels in growth chambers. Detailed growth stage-based phenotypic analysis will be performed to reveal different above and below-ground morphological and physiological responses of C3 and C4 grasses to enhanced CO2 levels condition. Also, the whole plant phenotypic observations will be complemented with the stomatal density, stomatal area, epidermal cell area and omics data to further link the observed phenotypic traits in the organismal level to tissue and molecular levels.
Project Details
Start Date
2017-07-14
End Date
2019-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Ahkami A., R.A. White, P. Handakumbura, and G.C. Jansson. 2017. "Rhizosphere Engineering: Enhancing Sustainable Plant Ecosystem Productivity in a Challenging Climate." Rhizosphere 3, no. 2:233-243. PNNL-SA-124740. doi:10.1016/j.rhisph.2017.04.012
Ahkami A., W. Wang, T.W. Wietsma, T.E. Winkler, I. Lange, G.C. Jansson, and M.D. Lange, et al. 2019. "Metabolic shifts associated with drought-induced senescence in Brachypodium." Plant Science Journal. PNNL-SA-145496. doi:10.1016/j.plantsci.2019.110278
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Fang Y., S.B. Yabusaki, A. Ahkami, X. Chen, and T.D. Scheibe. 2019. "A rhizosphere modeling capability to study the effect of root system architecture on soil water and reactive transport." Plant and Soil. PNNL-SA-138450. doi:10.1007/s11104-019-04068-z
Ogden, A.J., Wietsma, T.W., Winkler, T. et al. Dynamics of Global Gene Expression and Regulatory Elements in Growing Brachypodium Root System. Sci Rep 10, 7071 (2020). https://doi.org/10.1038/s41598-020-63224-z
Velickovic D., R.K. Chu, G.L. Myers, A. Ahkami, and C.R. Anderton. 2019. "An Approach For Visualizing The Spatial Metabolome Of An Entire Plant Root System Inspired by the Swiss-rolling Technique." Journal of Mass Spectrometry. PNNL-SA-142631. doi:10.1002/jms.4363