Fangjia Li
Chemist
Dr. Fangja Li is a chemist with the Cell Signaling and Communications team. Li focuses on quantitative fluorescence microscopy and single-cell analysis at the molecular level. Recently, he is using single molecule-based fluorescence imaging techniques, such as fluctuation localization imaging-based fluorescence in situ hybridization (fliFISH), using stochastic optical reconstruction microscopy principles, to understand cellular response to LCO nanoparticles at the molecular level and heterogeneous expression patterns of marker genes in pancreatic islet cells.
During Li’s PhD, he worked on femtosecond laser measurement and four-wave maxing at the Shanghai Institute of Optics and Fine Mechanics, and at Tongji University. Afterward, he studied the mechanical system of cells using FRET-based biological tension sensors with self-upgraded fluorescence lifetime imaging microscopy at the South Dakota School of Mines and Technology and Indiana University-Purdue University in Indianapolis, Indiana.
Research Interests
- Nonlinear optics
- Femtosecond laser
- Super-resolution microscopy
- Light-sheet microscopy
- Fluorescence lifetime imaging microscopy
- Confocal microscopy
- Molecular and cellular biology
- Machine learning
- Deep learning
Education
PhD in Optics, Tongji University
Affiliations and Professional Service
The Center for Sustainable Nanotechnology
Patents
J. Liu, F. J. Li, and R. X. Li. “Method and apparatus for femtosecond laser pulse measurement based on transient-grating effect.” US Patent 9,488,525.
Publications
2020
Y. Fan, A. Jalali, A. Chen, X. Zhao, S. Z. Liu, M. Teli, Y. X. Guo, F. J. Li, J. R. Li, A Siegel, L. X. Yang, J Liu, S. S. Na, M. Agarwal, A. G. Robling, A. Nakshatri, B. Y. Li, and Hiroki Yokota, Skeletal loading regulates breast cancer-associated osteolysis in a loading intensity-dependent fashion. Bone Res 8, 9 (2020).
R. G. Hart, D. Kota, F. J. Li, D. R. Pardo, A. Price, K. L. Otterpohl, S. J. Smith, A. R. Dunn, J. Liu and I. Chandrasekar, Myosin II Tension Sensors Reveal Dynamic Changes in Force Generated Along The Actin Cytoskeleton in Live Cells. elife, (2020)(Summited).
Fangjia Li, Dehong Hu, Cailin Dieter, Charles Ansong, Lori Sussel , Galya Orr, Single molecule-based fliFISH validates radial and heterogeneous gene expression patterns in pancreatic islet β cells. Diabetes, (2020) (Summited)
2019
F. J. Li, Andy Chen, S. Z. Liu, A. Reeser, Y. Wang, Y. Fan, X. Y. Zhao, Mr. R. Prakash, D. Kota, B. Y. Li, H. Yokota and J. Liu, “Vinculin Force Sensor Detects Tumor-Osteocyte Interactions. Sci Rep-Uk 9, (2019).