Yen-Chen Liao
Postdoctorate Research Associate
Yen-Chen Liao is a postdoctorate research associate doing single-cell proteomics using the nanoPOTS system. He works on combining the techniques from the top-down proteomics and the nanoPOTS teams to perform spatial proteomics of rat brains, which significantly increased proteoform identification and was published in Molecular & Cellular Proteomics in 2023.
Liao’s current research focuses on affinity purification for target protein enrichment under the nanoPOTS system. He aimed to use antibody-conjugated beads to enrich target protein in low microgram level protein input.
Research Interests
- Top-down proteomics
- Bottom-up proteomics
- Chemoproteomics
- Affinity-based protein profiling
- Affinity purification mass spectrometry
Education
- PhD in analytical chemistry, National Taiwan University, 2020
- MS in chemistry, National Chi Nan University, 2012
- BS in chemistry, National Tsing Hwa University, 2008
Affiliations and Professional Service
- Postdoctorate Researcher, Institute of Chemistry, Academia Sinica, Taipei, Taiwan, 2020
Publications
2023
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Liao, Y.-C.; Fulcher, J. M.; Degnan, D. J.; Williams, S. M.; Bramer, L. M.; Veličković, D.; Zemaitis, K. J.; Veličković, M.; Sontag, R. L.; Moore, R. J.; Paša-Tolić, L.; Zhu, Y.*; Zhou, M.*, Spatially Resolved Top-Down Proteomics of Tissue Sections Based on a Microfluidic Nanodroplet Sample Preparation Platform. Molecular & Cellular Proteomics 2023, 22 (2), 100491.
2022
- Zhou, M.*; Fulcher, J. M.; Zemaitis, K. J.; Degnan, D. J.; Liao, Y.-C.; Veličković, M.; Veličković, D.; Bramer, L. M.; Kew, W. R.; Stacey, G.; Paša-Tolić, L., Discovery top-down proteomics in symbiotic soybean root nodules. Frontiers in Analytical Science 2022, 2.
- Veličković, D.*; Liao, Y.-C.; Thibert, S.; Veličković, M.; Anderton, C.; Voglmeir, J.; Stacey, G.; Zhou, M.*, Spatial Mapping of Plant N-Glycosylation Cellular Heterogeneity Inside Soybean Root Nodules Provided Insights Into Legume-Rhizobia Symbiosis. Frontiers in Plant Science 2022, 13.
- Wilson, J. W.; Bilbao, A.; Wang, J.; Liao, Y.-C.; Velickovic, D.; Wojcik, R.; Passamonti, M.; Zhao, R.; Gargano, A. F. G.; Gerbasi, V. R.; Pas̆a-Tolić, L.; Baker, S. E.; Zhou, M.*, Online Hydrophilic Interaction Chromatography (HILIC) Enhanced Top-Down Mass Spectrometry Characterization of the SARS-CoV-2 Spike Receptor-Binding Domain. Analytical Chemistry 2022, 94 (15), 5909-5917.